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Advanced Fluorescence Techniques in Research on Micellar Systems and Their Interactions with Biopolymers
Holínková, Petra ; Burgert, Ladislav (referee) ; Táborský, Petr (referee) ; Pekař, Miloslav (advisor)
The dissertation thesis deals with study of advanced steady-state and time-resolved fluorescence techniques, which can be used for study of micellar systems properties. Selected fluorescence techniques were used for characterization of Septonex and CTAB cationic micellar systems and theirs interactions with hyaluronan. Fluorescent probe pyrene was used for determination of critical micelle concentration (CMC) and micellar aggregation number of these surfactants. The changes of fluorescence behaviour of fluorescein and prodan were studied in wide concentration range of Septonex. Next chapter of thesis deals with study of Förster resonance energy transfer between perylene and fluorescein in Septonex and CTAB micellar solutions and the effect of hyaluronan addition to these systems. Also steady-state and time-resolved fluorescence anisotropy studies were used for research of the effect of hyaluronan addition to micellar solutions. The last chapter of this thesis is focused on photophysical behaviour of Prodan in different solutions (water, Septonex solutions below CMC, hyaluronan solution, Septonex micellar solution and Septonex micellar solution with hyaluronan), which was discussed on the basis of time-resolved emission spectra.
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Behaviour of hydrophilic fluorescent probe Oregon Green 488 in hydrogel environment
Lacko, Kata ; Venerová, Tereza (referee) ; Mravec, Filip (advisor)
The aim of this bachelor’s thesis is to study the behavior of a hydrophilic fluorescent probe named Oregon Green 488 in hydrogel environment. The hydrogel was created using sodium hyaluronan and CTAB (cetyl trimethylammonium bromide) as a cationic surfactant. Solution of 0.15 M NaCl was used as a solvent, which provides physiologic conditions in the sample. The behavior of the fluorescent probe was characterized by steady-state and time-resolved fluorescent measurements. To obtain the emission spectra of the probe and to find the emission maximum the method named TRES (time-resolved emission spectra) was used. Fluorescence intensity decay measurements as a function of wavelengths allowed to create deconvolution of the emission spectra, which provided information about the fluorescent lifetime and the probe’s states’ relative representation in the solution. Hydrogels were examined 5 days and 1 month after the preparation and the results were compared. Oregon Green 488 demonstrated one fluorescent lifetime in the hydrogel environment in the range, 4,14,45 nm in the fresh hydrogel and 4,14,6 nm in the stable hydrogel. The emission maximum was obtained at 525 nm in both cases. The fluorescent probe’s behavior in the hydrogel environment is stable, presumably there are no interactions occurring between the hydrogel and the probe. Furthermore, no interactions were recorded between the probe and hyaluronan.
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Aerobic training in the treatment of gait disorders in patients with Parkinson's disease
KOCÁBKOVÁ, Štěpánka
This bachelor thesis investigates the effect of aerobic training on gait disorders in patients with Parkinson's disease. The specific features of gait in this disease are short, shuffling steps, slowed movement, impaired balance and reduced upper limb synkinesis. All of these features can, apart from the application of medication, be influenced to some extent by regular exercise. The aim of this bachelor thesis was to design a suitable exercise unit for these patients and to describe the effect of aerobic training on gait disorders. The theoretical part is an introduction to the issue of Parkinson's disease. Subchapters describe the definition, incidence, research history, anatomy and physiology of the basal ganglia, etiology, clinical features, stages, diagnosis and treatment. Furthermore, there is compared gait analysis of Parkinson's disease patients with healthy individuals and what the term aerobic training means. The practical part is processed in the form of qualitative research. Based on the input examination of three patients diagnosed with Parkinson's disease, I created an exercise unit, which can be found in Appendix 3. After three months, I did an output examination and compared if the created exercise unit had a positive effect in gait disorders or not. From the results, the positive effect of aerobic training on gait disorder in Parkinson's disease patients could not be clearly demonstrated. For a valid result, research would need a larger number of patients. However, patients evaluated the exercise unit positively and I believe that this work could find further application, for example in the clinical practice of physiotherapists or as an educational material for patients with Parkinson's disease and their family members.
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The effect of a regular exercise on the health of patients with Parkinson´s disease
DUBNOVÁ, Veronika
The bachelor thesis deals with the influence of regular exercise on the health of patients suffering from Parkinson's disease. This disease still remains an incurable disease that affects the human central nervous system and leads to its irreversible damage. Until now, the treatment of the disease has only made it possible to alleviate individual symptoms in the long term and thus maintain the patient's quality of life. An important part from the early stages of the disease is not only a pharmacological treatment but also a rehabilitation. The theoretical part of this work deals with a summary of information about Parkinson's disease, its origin, clinical picture, diagnosis and treatment options. The practical part of the work was processed by the method of qualitative research, which was done on the basis of an input and output examination, medical history taking, an interview and an evaluation of patients' ability to walk. The obtained data were compiled in the form of case reports. The study group consisted of 3 patients with a diagnosis of Parkinson's disease, who regularly attended group exercises once a week. The first goal was to evaluate the patient's condition during regular group exercise and the condition after 8 weeks without a group exercise. The second goal was to create an exercise unit for patients suffering from Parkinson's disease. Due to the small number of probands studied, it is certainly not possible to state whether the regular exercise once a week was sufficient in the treatment of Parkinson's disease. However, it was obvious that the patients felt good during the exercise, it positively affected their psychosocial condition and also their condition. Although I did not come to clear conclusions, I presume that the chosen therapy was beneficial.
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Kinesiotherapy of patients with Parkinson's disease
Kadeřábková, Michaela ; Savková, Pavlína (advisor) ; Molnár, Petr (referee)
Author: Michaela Kadeřábková Department: Department of rehabilitation medicine Title of the master thesis: Kinesiotherapy of patients with Parkinson's disease Supervisor: Mgr. Pavlína Savková Number of pages: 81 Number of attachments: 6 The year of presentation: 2012 Key words: Parkinson's disease, rehabilitation, tremor, rigidity Bachelor thesis deals with in the theoretical part about the origin, clinical symptoms and especially the treatment of PD. The treatments focuses primarily on rehabilitation treatment and analyzes kinesitherapy and its possibilities. The practical part is devoted to treatment of patients who suffer from this disease. Kinesiotherapy in selected patients is concentrated on major problems and their physical shortcomings.
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Advanced Fluorescence Techniques in Research on Micellar Systems and Their Interactions with Biopolymers
Holínková, Petra ; Burgert, Ladislav (referee) ; Táborský, Petr (referee) ; Pekař, Miloslav (advisor)
The dissertation thesis deals with study of advanced steady-state and time-resolved fluorescence techniques, which can be used for study of micellar systems properties. Selected fluorescence techniques were used for characterization of Septonex and CTAB cationic micellar systems and theirs interactions with hyaluronan. Fluorescent probe pyrene was used for determination of critical micelle concentration (CMC) and micellar aggregation number of these surfactants. The changes of fluorescence behaviour of fluorescein and prodan were studied in wide concentration range of Septonex. Next chapter of thesis deals with study of Förster resonance energy transfer between perylene and fluorescein in Septonex and CTAB micellar solutions and the effect of hyaluronan addition to these systems. Also steady-state and time-resolved fluorescence anisotropy studies were used for research of the effect of hyaluronan addition to micellar solutions. The last chapter of this thesis is focused on photophysical behaviour of Prodan in different solutions (water, Septonex solutions below CMC, hyaluronan solution, Septonex micellar solution and Septonex micellar solution with hyaluronan), which was discussed on the basis of time-resolved emission spectra.
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An accelerometry of tremor in multiple sclerosis
Pavlíková, Jitka ; Řasová, Kamila (advisor) ; Mende, Karel (referee)
Public health is largely devoted to preventive medicine. Therefore this thesis focuses on a topic that can affect the consequences caused by the tremor in the case of multiple sclerosis. The aim of the thesis is to elaborate the fundamental knowledge of the process of this tremor. The thesis is structured as follows. First it deals with the general characteristics of the tremor and the specific characteristics of the tremor in multiple sclerosis. It also discusses the possibilities of testing the tremor, where the largest part relates accelerometric examination using an accelerometer on the basis of which can provide useful information to both patients with the tremor and provide better coexistence with this symptom. Finally the thesis describes the therapeutic procedures that help to alleviate the tremor, which is an integral part of the ergotherapy. Powered by TCPDF (www.tcpdf.org)
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Kinesiotherapy of Parkinson`s disease and Parkinson`s syndrom
Zechovská, Lenka ; Vondráková, Ivana (advisor) ; Molnár, Petr (referee)
Author: Lenka Zechovská Institution: Rehabilitation Clinic, Faculty of Medicine in Hradec Králové Title: Kinesiotherapy of Parkinson's disease and Parkinson's syndrome Supervisor: Mgr. Ivana Vondráková Number of pages: 115 Number of attachments: 8 Year of defence: 2013 Keywords: Parkinson's disease, basal ganglia, tremor, rigidity, hypokinesia Bachelor thesis deals with the problems of Parkinson's disease and Parkinson's syndrome. The theoretical part includes the basal ganglia pathophysiology, etiopathogenesis and clinical manifestations of the disease, the possibility of diagnosis and treatment of disease. Acquaints with procedures of rehabilitation treatment, with specific methods and their application by geriatric patients. The practical part consists 2 case reports of patients. The first patient suffers from Parkinson's disease and the second one suffers from Parkinson's syndrome. Patients underwent a 4 and 5-week rehabilitation treatment at Home for the elderly in Dvůr Králové nad Labem.
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Kinesiotherapy of patients with Parkinson's disease
Kadeřábková, Michaela ; Savková, Pavlína (advisor) ; Molnár, Petr (referee)
Author: Michaela Kadeřábková Department: Department of rehabilitation medicine Title of the master thesis: Kinesiotherapy of patients with Parkinson's disease Supervisor: Mgr. Pavlína Savková Number of pages: 81 Number of attachments: 6 The year of presentation: 2012 Key words: Parkinson's disease, rehabilitation, tremor, rigidity Bachelor thesis deals with in the theoretical part about the origin, clinical symptoms and especially the treatment of PD. The treatments focuses primarily on rehabilitation treatment and analyzes kinesitherapy and its possibilities. The practical part is devoted to treatment of patients who suffer from this disease. Kinesiotherapy in selected patients is concentrated on major problems and their physical shortcomings.
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Behaviour of hydrophilic fluorescent probe Oregon Green 488 in hydrogel environment
Lacko, Kata ; Venerová, Tereza (referee) ; Mravec, Filip (advisor)
The aim of this bachelor’s thesis is to study the behavior of a hydrophilic fluorescent probe named Oregon Green 488 in hydrogel environment. The hydrogel was created using sodium hyaluronan and CTAB (cetyl trimethylammonium bromide) as a cationic surfactant. Solution of 0.15 M NaCl was used as a solvent, which provides physiologic conditions in the sample. The behavior of the fluorescent probe was characterized by steady-state and time-resolved fluorescent measurements. To obtain the emission spectra of the probe and to find the emission maximum the method named TRES (time-resolved emission spectra) was used. Fluorescence intensity decay measurements as a function of wavelengths allowed to create deconvolution of the emission spectra, which provided information about the fluorescent lifetime and the probe’s states’ relative representation in the solution. Hydrogels were examined 5 days and 1 month after the preparation and the results were compared. Oregon Green 488 demonstrated one fluorescent lifetime in the hydrogel environment in the range, 4,14,45 nm in the fresh hydrogel and 4,14,6 nm in the stable hydrogel. The emission maximum was obtained at 525 nm in both cases. The fluorescent probe’s behavior in the hydrogel environment is stable, presumably there are no interactions occurring between the hydrogel and the probe. Furthermore, no interactions were recorded between the probe and hyaluronan.
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